Harvard University

Advanced Technologies Core

CFAR Web Page » Lab Core Web Link »

Contact Information

Keith Reeves, HU CFAR Advanced Technologies Core Director

rreeves@bidmc.harvard.edu

617-735-4586

Galit Alter, HU CFAR Advanced Technologies Core Associate DirectorHU CFAR Program in HIV Vaccines Associate Director

galter@partners.org

Thomas J. Diefenbach

HU CFAR Advanced Technologies Core Associate Director, Microscopy

tdiefenbach@partners.org

Michael Waring

HU CFAR Advanced Technologies Core Associate Director, Flow Cytometry

mwaring@partners.org


Tests

Tests Flow Cytometry
Flow Cytometry (9 cytometers with up to 18 color analysis)
Cell Sorting (BD FACS Aria III at BSL2+ and BSL3)
Mass Cytometry (CyTOf)
Imaging Cytometry (Imagestream X Mark II)
Protein Interactions/Kinetics
Luminex Technology

 

Tests Microscopy
Zeiss LSM510 laser scanning confocal inverted microscope
Zeiss Axio Observer Z1 Automated Microscope
Zeiss Axio Observer A1 manual fluorescence microscope
TissueFAXS Whole Slide Scanning System
Zeiss MIRAX MIDI Slide Scanner
Image Analysis.

Tests Virology
Assists investigators with questions pertaining to the immune control of HIV, HCV, nonhuman primate immunodeficiency viruses, as well as other viruses that impact immuno-suppressed individuals. Sorting capabilities include the ability to sort live, infectious cells in a biosafety cabinet in a BSL2+ laboratory. Imaging capabilities include the ability to image live, infectious cells on a confocal microscope in a BSL2+ laboratory and up to 18 color flow cytometry for infectious samples.