Flow Cytometry Subcore
Stephen De Rosa, MD.
sderosa@fredhutch.org
206-667-1681
Humoral Immunology Subcore
Noah Sather, PhD
noah.sather@cidresearch.org
206-256-7229
Specialized Cellular Immunity Subcore
Ana Gervassi, PhD
ana.gervassi@cidresearch.org
206-256-7198
Non-Human Primate Immunology Subcore
Deborah Fuller, PhD.
fullerdh@wanprc.org
206-221-2328
HIV Co-Morbidities Subcore
Thomas Hawn, MD, PhD.
thawn@uw.edu
206 616-4124
Flow Cytometry Subcore
The mission of the flow cytometry subcore is to support all phases of experimental studies using flow cytometry including experimental design, staining panel design, laboratory procedures, collection of samples on the instrument, analysis of data and interpretation of results.
Assay for Cell Proliferation, Cell Sorting, Cytokine Bead Array, Experimental Design,
Humoral Immunology Subcore
The Humoral Immunity subcore offers the following services: antibody and antigen testing, recombinant protein and antibody production, viral and cell line culture, and neutralization assays.
Antibody-based Analyses for Antibodies or Antigens
Recombinant Protein and Antibody Production and Purification
Virus and Cell Line Culture and Neutralization Assays
Specialized Cellular Immunity Subcore
The Specialized Cellular Immunoassay sub-core aims to train individuals in state-of-the-art functional immunoassays. We can aid in assay design, optimization and validation of new assays as well as provide training for: T cell functional assays (measures of proliferation, cytotoxicity, cytokine secretion, viral suppression, etc) and NK functional assays (cytotoxicity, cytokine secretion, viral suppression, ADCVI).
Immunophenotyping and Functional Flow Cytometry Panels, NK Cell Function Assays, T Cell Function Assays
Non-Human Primate Immunology Subcore
Mucosal immunity as it relates to HIV prevention and eradication can be more extensively studied using animal models that allow for direct access to multiple tissues. The Non-Human Primate (NHP) Immunology Sub-core brings the following expertise:
a. Cellular immunology
Multiparameter flow cytometry to analyze cell subsets and T cell functions; ELISpot assays to map T and B cell responses to specific viral sequences.
Assessment of innate responses post SIV exposure
b. Mucosal immunology
Optimization of mucosal immunology assays including mucosal specimen collection in nonhuman primates
c. HIV vaccine study design, efficacy, and immunogenicity studies in nonhuman primates
Expertise in the analysis of immune correlates of protection or viral control in the blood, gut, and lung of nonhuman primates.
d. Flow cytometry core activities specific for nonhuman primate immunology.
HIV Co-Morbidities Subcore
The new HIV co-morbidities sub-core adds a number of immunological assays mainly focused on Mycobacterium tuberculosis(Mtb) and innate immunity such as:
a. Innate Immunity Expertise
Functional studies of monocytes, macrophages, and dendritic cells including: stimulation with TLR/NLR ligands or Mtb and measurement of cytokine secretion, effector function (such as NO production), and phagocytosis.
RNA analysis, including microarray experiments
Analysis and measurement of DC maturation and activation (flow cytometry)
Signaling assays in reconstitution systems, often with luciferase expressing reporter genes.
b. M. tuberculosis
Growth of Mtb and other mycobacteria (BCG, M.avium)
Infection of monocytes and macrophages with Mtb and measurement of cellular responses including replication (with lux expressing mycobacteria)
Mouse models of Mtb infection including assays measuring T cell mediated immunity.
